Frequent Detection of Human Coronaviruses in Clinical Specimens from Patients with Respiratory Tract Infection by Use of a Novel Real-Time Reverse-Transcriptase Polymerase Chain Reaction
Identifieur interne : 005571 ( Main/Exploration ); précédent : 005570; suivant : 005572Frequent Detection of Human Coronaviruses in Clinical Specimens from Patients with Respiratory Tract Infection by Use of a Novel Real-Time Reverse-Transcriptase Polymerase Chain Reaction
Auteurs : Leontine J. R. Van Elden [Pays-Bas] ; Anton M. Anton M. [Pays-Bas] ; Floris Van Alphen [Pays-Bas] ; Karin A. W. Hendriksen [Pays-Bas] ; Andy I. M. Hoepelman [Pays-Bas] ; Marian G. J. Van Kraaij [Pays-Bas] ; Jan-Jelrik Oosterheert [Pays-Bas] ; Pauline Schipper [Pays-Bas] ; Rob Schuurman [Pays-Bas] ; Monique Nijhuis [Pays-Bas]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2004.
Abstract
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens associated with more-severe respiratory tract infection (RTI). Diagnostic tests for HCoVs are not frequently used in the routine setting. It is likely that, as a result, the precise role that HCoVs play in RTIs is greatly underestimated. We describe a rapid, sensitive, and highly specific quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of HCoV that can easily be implemented in the routine diagnostic setting. HCoV was detected in 28 (11%) of the 261 clinical specimens obtained from patients presenting with symptoms of RTI ranging from common cold to severe pneumonia. Only 1 (0.4%) of the 243 control specimens obtained from patients without symptoms of RTI showed the presence of HCoV. We conclude that HCoVs can be frequently detected in patients presenting with RTI. Real-time RT-PCR provides a tool for large-scale epidemiological studies to further clarify the role that coronavirus infection plays in RTI in humans.
Url:
DOI: 10.1086/381207
Affiliations:
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R." last="Van Elden">Leontine J. R. Van Elden</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Pays-Bas</country></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Pays-Bas</country><wicri:regionArea>Reprints or correspondence: Dr. Leontine J. R. van Elden, Eijkman-Winkler Center for Microbiology, Infectious Diseases and Inflammation, Dept. of Virology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht</wicri:regionArea><wicri:noRegion>3508 GA Utrecht</wicri:noRegion></affiliation></author><author><name sortKey="Anton M, Anton M" sort="Anton M, Anton M" uniqKey="Anton M A" first="Anton M." last="Anton M.">Anton M. Anton M.</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Van Alphen, Floris" sort="Van Alphen, Floris" uniqKey="Van Alphen F" first="Floris" last="Van Alphen">Floris Van Alphen</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Hendriksen, Karin A W" sort="Hendriksen, Karin A W" uniqKey="Hendriksen K" first="Karin A. W." last="Hendriksen">Karin A. W. Hendriksen</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Hoepelman, Andy I M" sort="Hoepelman, Andy I M" uniqKey="Hoepelman A" first="Andy I. M." last="Hoepelman">Andy I. M. Hoepelman</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Internal Medicine, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Van Kraaij, Marian G J" sort="Van Kraaij, Marian G J" uniqKey="Van Kraaij M" first="Marian G. J." last="Van Kraaij">Marian G. J. Van Kraaij</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Hematology, University Medical Center St. Radboud, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Oosterheert, Jan Jelrik" sort="Oosterheert, Jan Jelrik" uniqKey="Oosterheert J" first="Jan-Jelrik" last="Oosterheert">Jan-Jelrik Oosterheert</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Internal Medicine, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Schipper, Pauline" sort="Schipper, Pauline" uniqKey="Schipper P" first="Pauline" last="Schipper">Pauline Schipper</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Schuurman, Rob" sort="Schuurman, Rob" uniqKey="Schuurman R" first="Rob" last="Schuurman">Rob Schuurman</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author><author><name sortKey="Nijhuis, Monique" sort="Nijhuis, Monique" uniqKey="Nijhuis M" first="Monique" last="Nijhuis">Monique Nijhuis</name><affiliation wicri:level="3"><country>Pays-Bas</country><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><wicri:orgArea>Departments of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht</wicri:orgArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date type="published">2004</date><biblScope unit="vol">189</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="652">652</biblScope><biblScope unit="page" to="657">657</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens associated with more-severe respiratory tract infection (RTI). Diagnostic tests for HCoVs are not frequently used in the routine setting. It is likely that, as a result, the precise role that HCoVs play in RTIs is greatly underestimated. We describe a rapid, sensitive, and highly specific quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of HCoV that can easily be implemented in the routine diagnostic setting. HCoV was detected in 28 (11%) of the 261 clinical specimens obtained from patients presenting with symptoms of RTI ranging from common cold to severe pneumonia. Only 1 (0.4%) of the 243 control specimens obtained from patients without symptoms of RTI showed the presence of HCoV. We conclude that HCoVs can be frequently detected in patients presenting with RTI. Real-time RT-PCR provides a tool for large-scale epidemiological studies to further clarify the role that coronavirus infection plays in RTI in humans.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre</li><li>Utrecht (province)</li></region><settlement><li>Nimègue</li><li>Utrecht</li></settlement></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Van Elden, Leontine J R" sort="Van Elden, Leontine J R" uniqKey="Van Elden L" first="Leontine J. R." last="Van Elden">Leontine J. R. Van Elden</name></region><name sortKey="Anton M, Anton M" sort="Anton M, Anton M" uniqKey="Anton M A" first="Anton M." last="Anton M.">Anton M. Anton M.</name><name sortKey="Hendriksen, Karin A W" sort="Hendriksen, Karin A W" uniqKey="Hendriksen K" first="Karin A. W." last="Hendriksen">Karin A. W. Hendriksen</name><name sortKey="Hoepelman, Andy I M" sort="Hoepelman, Andy I M" uniqKey="Hoepelman A" first="Andy I. M." last="Hoepelman">Andy I. M. Hoepelman</name><name sortKey="Nijhuis, Monique" sort="Nijhuis, Monique" uniqKey="Nijhuis M" first="Monique" last="Nijhuis">Monique Nijhuis</name><name sortKey="Oosterheert, Jan Jelrik" sort="Oosterheert, Jan Jelrik" uniqKey="Oosterheert J" first="Jan-Jelrik" last="Oosterheert">Jan-Jelrik Oosterheert</name><name sortKey="Schipper, Pauline" sort="Schipper, Pauline" uniqKey="Schipper P" first="Pauline" last="Schipper">Pauline Schipper</name><name sortKey="Schuurman, Rob" sort="Schuurman, Rob" uniqKey="Schuurman R" first="Rob" last="Schuurman">Rob Schuurman</name><name sortKey="Van Alphen, Floris" sort="Van Alphen, Floris" uniqKey="Van Alphen F" first="Floris" last="Van Alphen">Floris Van Alphen</name><name sortKey="Van Elden, Leontine J R" sort="Van Elden, Leontine J R" uniqKey="Van Elden L" first="Leontine J. R." last="Van Elden">Leontine J. R. Van Elden</name><name sortKey="Van Elden, Leontine J R" sort="Van Elden, Leontine J R" uniqKey="Van Elden L" first="Leontine J. R." last="Van Elden">Leontine J. R. Van Elden</name><name sortKey="Van Kraaij, Marian G J" sort="Van Kraaij, Marian G J" uniqKey="Van Kraaij M" first="Marian G. J." last="Van Kraaij">Marian G. J. Van Kraaij</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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